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An analysis of GC–MS data was conducted on extracts of Hybanthus enneaspermus to determine the bioactive compounds. Over ten compounds were detected, including 2-(Acetoxymethyl)-3-(methoxycarbonyl)biphenylene, 2-Ethylacridine, beta-Sitosterol acetate, 4-(Diethylaminomethyl)-2,5-dimethylphenol, Methyl 8,11,14-heptadecatrienoate, Spiro[4.5]dec-6-ene, 4-Dehydroxy-N-(4,5-methylenedioxy-2-nitrobenzylidene)tyramine, 2,6-dimethyl-nonanoic acid and 5-Butyl-6-hexyloctahydro-1H-indene. It appears that the methanol extract of Hybanthus enneaspermus revealed a greater number of compounds. Specifically, we sought to isolate the compound that may possess various biological properties as part of our further investigation.