Measurement of Some Semen Parameters Stored at 5°C for Rams after Adding Different Levels of Artichoke (Cynara Scolymus L.) Aqueous Extract

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Zina Rashid Khirija AL-Badri, Husam Jasim Hussein Banana

Abstract

This study was conducted in the animal field and laboratories of the Department of Animal Production/College of Agricultural Engineering Sciences /University of Baghdad in Jadiriyah during the period from November 15, 2021 to April 25, 2022. Three local Awassi rams were used in this experiment, their ages ranged between 2.5-3.0 years. The semen was mixed with the artificial vagina by one ejaculate / ram / week, and for a period of 8 weeks, the ram’s semen was mixed in order to remove the individual differences, and then the following tests were performed in order to evaluate the semen characteristics for each of the total abnormalities, acrosome integrity and the integrity of the plasma membrane with a procedure Biochemical tests, which included studying the concentration of MDA compound and the concentration of total antioxidants, this study was conducted to show the effect of adding aqueous extract of the leaves of the artichoke plant Artichoke ( Cynara scolymus) to Tris extender and its effect on the semen characteristics of Awassi rams after preservation at cooling of 5 °C. ˚ and for different durations (0, 24, 48 and 72 hours). The results of the current study showed that the addition of aqueous extract of the leaves of the artichoke plant ( Cynara Scolymus) led to an improvement in most of the studied traits during preservation. The results of the percentage of distorted sperm at the 48-hour preservation period indicated a significant superiority (P<0.01) for T1 and T2 treatments, which amounted to 14. 12) ± 0.76 and 14.75 ± 0.72%) compared to the control treatment (C), which amounted to (17.25 ± 0.70%), while it did not differ significantly with treatments and T3 and T4, which amounted to (15.50 ± 0.37 and 15.37 ± 0.90%), while it showed Results There were no significant differences between treatments during preservation periods (0, 24 and 72 hours) compared to control treatment (C) in the percentage of deformed sperm for Awassi rams. (0, 24, 48 and 72 hours) in the integrity of the plasma membrane and the integrity of the peripheral body. As for measuring the biochemical characteristics of seminal plasma, the results showed an arithmetic decrease in the concentration of total antioxidants for treatments T1, T2, T3 and T4 compared with the control treatment (C) after 72 hours. An hour of preservation. The results also showed an arithmetical superiority in the concentration of malon didehyde after 72 hours of preservation for treatments T1, T2, T3, and T4 compared with the control treatment.

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