A Validated RP-HPLC Method for Simultaneous Estimation of Clobetasol and Nadifloxacin in Tablet Dosage form

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M. Hima Bindu, G. Shiva Kumar


Various trials were conducted to establish a simple, precise, accurate, robust, and cost-effective approach for regular Clobetasol and Nadifloxacin analysis. The estimation was performed on a 5m, 250mm4.6mm i.d. Enable C-18 column. As the mobile phase, a 70:30 mixture of acetonitrile and 0.5 percent potassium dihydrogen phosphate buffer (adjusted to pH-5 using orthophosphoric acid) was utilised. The flow rate has been set to 1.0ml/min. Clobetasol and Nadifloxacin linearity was in the 10-100g/ml and 10-100g/ml ranges, respectively. Clobetasol and Nadifloxacin mean recoveries were determined to be 99.2 to 100.3 percent and 99.7 to 100.3 percent, respectively.

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