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This study was aimed to determine the antimicrobial, toxicity, and anticancer activities of ethyl acetate extracts from sponge-associated bacteria and to detect the presence of PKS and NRPS genes.Infectious diseases and cancer are important to a health problem. Infectious diseases are caused by pathogenic microorganisms, such as bacteria, viruses, parasites or fungi. Each year infectious diseases kill 3.5 million people. Cancer is a generic term for a large group of diseases that can affect any part of the body. Cancer is a leading cause of death worldwide, accounting for 8.2 million deaths in 2012. Therefore the search for novel therapeutic agents continues, and the need still remains to uncover the initial structural lead that interact with therapeutic targets. Natural products give a good chance for the discovering an effective medication of the remaining untreatable diseases.