A Phenotypic and Molecular Study of Colistin Resistance among Members of the Intestinal Family Isolated From Clinical Cases in Al-Diwaniyah City

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Luqaa Talib Amin, Azhar Noory Hussein

Abstract

200 clinical samples were collected from patients suffering from different infections, in different age groups, for both sexes, and from different sources.


It was distributed among 63 (Urine) samples, 58 (Sputum) samples, 42 (Stool) samples, and 37 (Burns) and (Wounds) patients from the patients visiting and sleeping in Al-Diwaniyah Teaching Hospital and the Children’s and Maternity Hospital in Al-Diwaniyah, whose ages ranged between (1-60) years. For a period of three months, from 9/15/2021 to 01/10/2022.


     The isolates were diagnosed based on phenotypic, microscopic and biochemical tests, and the diagnosis was confirmed using the Vitek 2 system, in addition to molecular diagnostics to investigate the SrRNA16 gene. genes. The results of isolation showed that 141 samples (70.5%) had positive bacterial growth, while 59 samples (29.5%) had negative bacterial growth. The growing samples were distributed among 87 samples (79%) of females compared to 54 samples (60%) of males. The results showed that 30 isolates of E.coli, 20 isolates of Salmonella enterica, 15 isolates of Klebsiella pneumonia, and 10 isolates of Proteus mirabilis and Enterobacter cloacae.


     The susceptibility of samples to colistin resistance was investigated by means of a sensitivity test using Muller-Hinton culture medium and by using the disc method, the result was that all samples were 100% colistin resistant. , mcr-2) chromosomal and plasmid using PCR technique, as the results showed that the percentage of the chromosomal mcr-1 gene is 52.9% and the plasmid by 17.6%, while the mcr-2 gene appeared in a percentage of 52.9% chromosomal and 64.7% plasmid, and it was shown from the results shown above All samples carried the mcr gene, and for this reason they had a high resistance to colistin.

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