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Objectives: Acinetobacter baumannii is a gram-negative, non-lactose fermenting organism that is increasingly recognized as a major pathogen causing nosocomial infections .so this study aimed to evaluate about 200 sample from different source and 86 isolates diagnosed biochemically as A .baumannii, while 50 strains with MDR and XDR from A . baumannii amplified for antibiotic resistant genesby polymerase chain reaction (PCR)method and finally sequenced the 16SRNA and NDM-2 gene.
Methods: Fifty of A .baumannii out of 200 clinical samples was isolated from different sources (burns, sputum, wound swabs and urine) in both sexes (31) male and (19) female. These isolates were diagnosed by many media and VITEK -2 system. The bacterial DNA of A. baumannii isolatewere extracted from 50 isolates with MDR and XDR of A. baumannii. Molecular Detection of 16S rRNA and antibiotic resistant gene (NDM-2, tet–B) genes with make sequencing for 16SrRNA and NDM-2 gene were performed
Results: The result of amplification 16S rRNA from bacterial isolate revealed that the all isolate 50 isolates were amplified and about (32) isolate have positive for 16SrRNA gene and Also detection of antibiotic resistance genes (NDM-2,and tet (B),and blaTEM gene were done by polymerase chain reaction for fifty isolates of A .baumannii.with number (37), (27) and(22) respectively. Three isolates ( one from burn and two sample sputum from covid patient ) were identified as A. baumannii by 16srRNA sequence analysis. Result of Sequencing (NDM-2 and 16SrRNA)of A. baumannii the compatibility between study isolates appeared no mutation occur with identity (100%).