Screening of Terminalia Chebula Carp Extract for Biologically Active Compound against Fusarium Oxysporum Spp. (WILT) and Its Toxicity on Tomato Germination and Growth

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Padmalatha A, Dr. Prabha SB

Abstract

Terminalia chebula well known medicinal plant with a wide range of applications in many fields. Its leaves, bark and fruits were explored for medicinal purposes. In this study water, ethanol (Polar), benzene and chloroform (nonpolar) organic solvents were used to extract the chemical compound from the dried fruit of Terminalia chebula carp by cold extraction. Biological activity of extracted compounds tested against plant pathogenic fungi Fusarium oxysporum. Standard culture of Fusarium oxysporum ITCC -6246, Fusarium oxysporum f. sp. ciceri ITCC 3636 and Fusarium oxysporum f. sp. Lycopersici MTCC 10270 were used to detect the potential chemical compound in dried fruit by macro-dilution. The chemical compounds present in these extracts were determined by the preliminary phytochemical test and confirmed the presence of tannins, phenolic compounds, coumarins and saponins, further analyzed by GCMS. An in-vitro test performed on the spores of Fusariumspp. in liquid medium by macro-dilution method and IC 50 was found to be 5mg/ml for water and benzene extract, 1-2mg/ml for ethanol extract and 2mg/ml of chloroform extract for Fusarium oxysporum. Rate of spore germination inhibition of each extract was calculated from the mean value of triplicate for every solvent extract used. IC 70 was calculated and analyzed statistically by t-test for significant activity. The efficient solvent extract tested for the biologically active compound from carp by invivo on the seeds of model plant tomato for any toxic effect. But the results confirm the absence of such toxic compounds instead it promotes seed germination and growth. 

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