Study Effect of Silver Nanoparticles on the Parasite Cryptosporidium Spp. Isolated from Water Sources in Experimentally Laboratory Animals Infected

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Asma Hussain Jaber, Sadiya Aziz Anah

Abstract

 Background: The cryptosporidium parasite is an intestinal eukaryotic protozoan that parasitizes inside cells and infects humans and animals, causing cryptosporidiosis. Research confirms that cryptosporidium parasite is one of the causes of gastroenteritis that accompanies diarrhea in humans and animals for this reason.  It is considered one of the common pathogens of humans and animals Caccio (2005).


 Objective: To find out the effect of silver AgNPS nanoparticles on the cryptosporidum parvum parasite isolated from different water sources by infecting laboratory white mice of type Balb/C with Cryptosporidium parasite at a dose of 1×103 oocytes/lab rat, where each group was given 1 ml of the specified dose and then dosed in concentrations  different from AgNPS.


 Methods of work: Water samples were collected during the period from September 2021 to February 2022, and 500 water samples were distributed among three different water sources.  The oocytes of cryptosporidum parvum were isolated and purified by using the flotation method with sugar cheetah solution. Laboratory mice were also used in this study and dosed at a dose of 1 x 310 oocytes/lab mouse, and the delay time was recorded (4_5) i.e. days, where the infection rate was recorded for laboratory mice at a rate of 100%.


Results: The results of the current study indicated that the total percentage of contamination of the three water sources (rivers, purification plants and liquefaction water) with C. spores was 16%.


     The results of this study also indicated the use of AgNPS as a treatment for mice infected with oocysts of the parasite C. parvum for a period of one week, the effect of the three concentrations (10, 20, 30) mg/kg varied in reducing or reducing the number of oocysts excreted with the feces of experimentally infected mice,


 Conclusions: The concentration of 30 mg/kg of AgNPS was the best concentration, as it completely reduced the ovarian cysts on the fifth day and with a therapeutic efficiency of 100%, and the concentration was closest to the group treated with Nitazoxanid, which achieved 100% therapeutic efficiency on the fourth day of treatment.


    As for the concentration of 20 mg/kg, it achieved a therapeutic efficacy on the sixth day of treatment with a percentage of 100%, while the mice treated with a concentration of 10 mg/kg continued to excrete the oocytes until the seventh day of treatment.


     This study also showed histological changes in the intestines of infected mice, including atrophy, villous necrosis, hyperplasia, and the detachment of some villi from their bases and their clear destruction. As for the tissue sections prepared from the liver of mice dosed with the same concentration of 30 mg/kg, if most of the sections were observed in a normal condition except for one sample, it appeared  It has congestion in the portal vein.

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