An in Vitro Detection of Antibacterial Inhibitory Effect of Aqueous Extract of Melissa Officinalis and Plantago Ovate

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Marwa Kadhim Khudhair, Zainab Adil Ghani Chabuck, Nada Khazal Kadhim Hindi, Abduladheem Turki Jalil

Abstract

Background:There is an increment in the public alarm about hygiene that stimulates many works and researches to inspect and improve different antimicrobial agents. However, the applications of various antimicrobial reagents must be taperedbecause of their possible harmful or toxic effects. Also, the usage of plants materials as an alternative of chemical or synthetic drugs is increased in last years, in order to reduce unwanted effects of these antibiotics. WHO informed that about 80% of population around the world still trusting on plants and their extracts as sources for principal health care, despite the fact traditional medication is the only available health source for more than 60% of the world'spopulation?


Aim: to evaluate the inhibitory effects of Melissa officinalis and Plantago ovata aqueous extracts, as growth inhibitors and /or inhibition of other physiological behaviors as surface adhesion and biofilm formation.


Material and Methods: Aqueous extracts of the mentioned plants were applied to examine their effects for inhibition of growth and some physiological parameters as adhesion and biofilm-formation against 19 bacterial isolates (both positive and negative clinical isolates). Agar wells-diffusion method, biofilm inhibition test by the using of tissue culture plate method and adherence inhibition assay were done for the estimation and evaluation the inhibitory effects.


Results:aquatic extract ofMelissa officinalisexpressing significant antibacterial inhibitory activity against Gram positive more than Gram negative bacteria, especially against Strep. faecalis with an inhibition zone 36mm.the aquatic extracts of Melissa officinalis gave high inhibitory effects for adhesion and biofilm formation against all the tested bacteria; in comparison with the inhibitory effect the extract ofPlantago ovata.


Conclusion:Medicinal plants act as therapeutic agents that inhibit the growth of pathogens considered as potential antimicrobial compounds, as these compounds are cheap, available and broadly-effective against wide range of severe human pathogens, that causing dangerous diseases and complications.

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