Assessment of Phytochemicals, Antioxidant and Antimicrobial Efficacy from Different Solvent Extracts of a Medicinal Plant – Of Cyanthilliumcinerium(L.) H. Rob.

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Mohamud Maitheen.A, Janaki Devi.A, Prabha.S.B

Abstract

In this study, the antioxidant and antimicrobial potential of Cyanthilliumcinerium (L.) H. Rob. whole plant crude extract was investigated. Polar solvents (methanol, aqueous) and non-polar solvent (hexane) was used, to extract the biologically active components from the plant sample by adopting hot continuous soxhlet extraction method. Phytocompounds such as alkaloids, carbohydrates, saponins and phenolic compounds were chiefly identified in the methanol and aqueous extracts. The hexane extract showed only the presence of phenolic compounds. The quantity of total phenolic content was found to be high in methanol extract (4.45 mg/g), followed by aqueous extract (3.2 mg/g) and hexane extract (1.4 mg/g). Similarly, total flavonoids were high in methanol extract (1.20 mg/g). The quantity of flavonoid was (0.20 mg/g) and (0.10 mg/g) in the hexane and aqueous extracts respectively. Antioxidant radical scavenging activity conducted with DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and phosphomolybdenum assay for the crude solvent extracts showed potent results. The DPPH % inhibition expressed by methanol extract was IC50 27.23 µg/mL and ABTS % inhibition was found to be IC50 58.98 µg/mL. The free radical scavenging potential of methanol extract with the phosphomolybdenum assay showed highest absorbance of 1.211nm at 100 µg/mL. Aqueous extract possessed high ion chelating activity, IC50 value of 46.87 µg/mL. The crude extract of the plant was screened for its antimicrobial zone of inhibition against five human pathogenic organisms such as Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Methicillin resistant Staphylococcus aureus and Candida albicans. Maximum zone of antimicrobial inhibition of 5 mm and 3 mm was exhibited by 150 µg/mL of methanol extract followed by hexane extract against Pseudomonas aeruginosa and Methicillin resistant Staphylococcus aureus, respectively.

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