Patients with Acute Myocardial Infarction Exhibit Intestinal Flora Dysbiosis

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Lijun Wang, Jiakun Dong, Xinying Li, Yang Wang, Hujie Quan, Jun Yan, Aikun Wang

Abstract

Background: The relationship between intestinal flora dysbiosis and coronary heart disease (CHD) has recently attracted great attention.
Objectives: In order to explore the difference of composition in the intestinal flora between acute myocardial infarction (AMI) patients and pateints not suffering from coronary heart disease (non-CHD).
Methods: We enrolled 28 AMI patients aged from 51 to 70 as the test group, and 28 patients aged from 51 to 70 not suffering from coronary artery disease as the control group. Stool samples were collected from which bacterial DNA was extracted and analyzed by denaturing gradient gel electrophoresis (DGGE) after PCR amplification of the V3 region of the 16S rRNA gene. Cluster analysis of the DGGE patterns revealed that the AMI patients groups were separated from the control group except for the older female AMI patients (group D). All groups were submitted to an amplification of the 16S rRNA V3~V4 region, and high throughput sequencing using the Illumina Miseq platform. Results: The high throughput sequencing and Metastats analysis revealed that there are significant differences in the bacterial populations found in the intestinal flora of AMI patients, compared to non-CHD patients, at the phylum, class, order, family and genus levels.
Conclusions: The intestinal flora of the AMI group showed significant increase in pathogenic bacteria and a significant decrease in beneficial bacteria, strongly suggesting that intestinal flora dysbiosis may be closely related to the onset of AMI.

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