Genetic Polymorphisms of Interleukin-6 Gene Among Patients with Helicobacter pylori

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Aqeel Malik Salih, Orass Madhi Shaheed

Abstract

Objective:We investigated the connection between IL6 polymorphisms and Helicobacter pylori (H. pylori) infection because both variations in the interleukin-6 (IL6) gene and severe infection of the stomach mucosa caused by H. pylori are known to influence inflammation and gastric carcinogenesis.


Methods: Baghdad's Gastrointestinal Tract and Liver Diseases Teaching Hospital was used to collect blood samples from laboratories andfrom laboratories in Al-Karamah Teaching Hospital and in Wasit, that were under monitoring at the time. There were 50 patients with H. pylori infection related gastritis in the first group, which included (23 males and 27 females).The second group (controls) consisted of 50 people who tested negative for H. pylori (39 men and 11 females). Between November 2020 and March 2021, their ages ranged from 12 to 70. All of the samples were sent to the lab and kept at a temperature of -30°C. Blood was immediately drawn into a sterile tube containing EDTA for DNA extraction, which was then applied to IL-6 polymorphisms for molecular analysis employing the (ARMS- PCR) method.


Results:The present work, Detection of IL-6 (rs1800795) SNP, a polymorphism located about -174 in the 5′ flanking domain (G→C) also, the frequency of genotypes C\C was 93 (0.93) as 48(0.96) , 45(0.9) in control and patients, respectively and the frequency of genotype C\G was 7( 0.07) as 2(0.04) ,5(0.1) in control and patients, respectively. Finally the frequency of genotypes G\G was 0 (0) for control and patients.


Conclusion: The current study found no link between IL-6 gene polymorphisms and  a greater risk of illness in the presence of H. pylori.

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