Microrna-122: A Prognostic Marker of the Pathological State of the Liver

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Dinara P. A., Shukurova F. N., Salayeva M. S., Salimova N. Dj., Sibirkina M. V.

Abstract

This article provides information on the role of miRNAs in the progression of chronic diffuse diffuse liver diseases from the stage of chronic viral hepatitis to severe fibrosis. The study of new parameters for predicting the nature of the course, the rate of progression of liver fibrosis, the risk of developing HCC is of particular importance. In this direction, great prospects are opening up in the field of genomics. Among the total array of works, a significant share is occupied by studies of single nucleotide polymorphisms, freely circulating DNA, endosomal RNA and their significance in the pathogenesis of various diseases. The discovery in 2002 of microRNA-122 (miR-122) was an event in hepatology due to the functional features of this RNA cluster.


Purpose: to study the expression level of microRNA-122 as a prognostic parameter for the risk of complications in patients with chronic viral hepatitis.


Material and methods: to study the role of microRNA-122, 32 patients were selected from the total number of examined patients: 17 (53.1%) of them with a diagnosis of chronic viral hepatitis made up 1 subgroup and 15 (46.9%) with a diagnosis of compensated cirrhosis of the liver 2 subgroup. The expression level of microRNA-122 in blood plasma was measured using reverse transcription PCR in accordance with the TaqMan microRNA analysis protocol. The study corresponds to the priority areas for the development of science and technology in the Republic of Uzbekistan.


Results: Intervals of the miRNA-122 expression level range were determined, the division was four-level from the minimum values   of 0.001 - 0.14; 0.15 -1.05; 1.05 - 12.88 and >12.89. The lowest levels of miRNA-122 were found in patients with severe liver fibrosis, being indicators of the functional capacity of the liver. It has been established that in patients with chronic viral hepatitis and cirrhosis of the liver, the range of miRNA-122 expression levels varies depending on the stages of fibrosis;


It has been proven that a decrease in the expression level of miRNA-122 is a prognostic marker in the development of complications of chronic viral hepatitis. The prognostic criteria of microRNA-122 were assessed in relation to the unfavorable prognosis of the risk of complications.


Conclusion: the significance of the results of the study lies in the need to determine the level of miRNA-122 expression as a prognostic marker for the development of complications in chronic viral hepatitis and to include molecular genetic research methods in the standard of patient examination, which prevents early disability and reduces mortality.

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