Characterization and Antibacterial Activity of Aqueous Leaf Extract Mediated Green Synthesized Silver Nanoparticles Using Viola Serpens

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Ashish Kumar Rai, Payal Pandey, Anupam Singh, Ajay Kumar

Abstract

Silver nanoparticles(AgNPs) are being synthesised using a green approach, which is gaining popularity in the field of nano-science research. This study aimed to synthesize AgNPs using the leaves extract of Viola serpens and test antibacterial activity against Bacillus subtillis and Pseudomonas aeruginosa. The leaves of V.serpens were employed as a reducing agent in the production of silver nanoparticles(AgNO3) from an aqueous solution of silver nitrate, and the results were promising . The characterization of the synthesised AgNPs included UV-Vis spectroscopy, XRD (X-Ray Diffraction), SEM (Scanning Electron Microscope) with EDX (Energy Dispersive X-Ray Analyzer, and FTIR (Fourier Transform Infrared Spectroscopy). At wavelengths 430-440 nm, surface plasmon resonance spectroscopy was detected in the UV-VIS range. The XRD data shows that the NPs are crystalline in their natural state. The results of the EDX test revealed the presence of Ag metal. The size of the AgNPs, according to the SEM data, was between 30 to 40 nm in diameter. The data also supported the cubical form of the nanoparticles, which was hypothesised before. The results obtained from antibacterial activity test showed that the substantial suppression of the development of two bacteria (B.subtillis and P. aeruginosa), in the presence of the compounds like ascorbic acid, ascorbate oxidase, peroxidase catalse etc. The synthesized AgNPs were shown the 9 mm and 10 mm zone of inhibition against B. subtilis and P. aeruginosa respectively, while 1mM AgNO3 and plant extract of V. serpens have shown no zone of inhibition. It is also a viable alternative to conventional chemical and physical methods. At room temperature, just 15 to 30 minutes were necessary for the conversion of silver ions into silver nanoparticles, and no harmful chemicals were used in the process.In addition, the results indicated that this approach is environmentally friendly and non-toxic.

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